Table 1 Bacterial strains and plasmids used in this study

Strains

 E. coli

  DH5α

f80dlacZΔM15, Δ(lacZYA-argF)U169, deoR, recA1, endA1, hsdR17(rk−, mk+), phoA, supE44, l−, thi-1, gyrA96, relA1

Transgen

  BL21(DE3)

ompT gal dcm lon hsdSB(rB−mB−) l (DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5])

Transgen

 C. thermocellum

  DSM1313

Wild-type stain

DSMZ

  ΔpyrF

Derived from DSM1313, with deleted pyrF gene

[28]

  ∆pyrF::CaBglA

Derived from ΔpyrF, producing a fusion protein of Cel48S and CaBglA

[28]

  ∆pyrF::CaBglAm

Derived from ΔpyrF, producing a fusion protein of Cel48S and CaBglAm

[28]

  ΔpyrF::KBm

Derived from ΔpyrF, producing a fusion protein of Cel9K and CaBglA

This work

Plasmids

 pHK-HR-CaBglA

Seamless genome editing plasmid for markerless knock-in of caBglA in the chromosome of DSM 1313

[28]

 pHK-HR-KBm

Derived from pHK-HR-CaBglA, with different homology arms

This work