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Table 1 Bacterial strains and plasmids used in this study

From: Construction of consolidated bio-saccharification biocatalyst and process optimization for highly efficient lignocellulose solubilization

Strains/plasmids Relevant characteristic Sources
Strains
 E. coli
  DH5α f80dlacZΔM15, Δ(lacZYA-argF)U169, deoR, recA1, endA1, hsdR17(rk, mk+), phoA, supE44, l, thi-1, gyrA96, relA1 Transgen
  BL21(DE3) ompT gal dcm lon hsdSB(rBmB) l (DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5]) Transgen
 C. thermocellum
  DSM1313 Wild-type stain DSMZ
  ΔpyrF Derived from DSM1313, with deleted pyrF gene [28]
  ∆pyrF::CaBglA Derived from ΔpyrF, producing a fusion protein of Cel48S and CaBglA [28]
  ∆pyrF::CaBglAm Derived from ΔpyrF, producing a fusion protein of Cel48S and CaBglAm [28]
  ΔpyrF::KBm Derived from ΔpyrF, producing a fusion protein of Cel9K and CaBglA This work
Plasmids
 pHK-HR-CaBglA Seamless genome editing plasmid for markerless knock-in of caBglA in the chromosome of DSM 1313 [28]
 pHK-HR-KBm Derived from pHK-HR-CaBglA, with different homology arms This work