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Table 2 Primers used in this study

From: Construction of consolidated bio-saccharification biocatalyst and process optimization for highly efficient lignocellulose solubilization

Primers Sequences (5′–3′)a
Up-1 GTCTCTAGACATTGGCATTCTTCTATCACAAG (XbaI)
Up-2 GTCGTCGACTCCTCCTCCTGGCGGTGTTATT (SalI)
Short-1 GTCCTCGAGGGACTGGATCAGTCCTATG (XhoI)
Short-2 GTCCGGCCGTCCTCCTCCTGGCGGTGTTATT (EagI)
Down-1 GTCGCGCGCGGAGGAGGAGTAGACCCAGAAGAACCGGAG (MluI)
Down-2 GTCGGATCCACTACAACGCCTGCAGCATTC (BamHI)
  1. aRestriction sites are underlined and indicated in following parentheses