Fig. 3
Ploidy determination, thermotolerant phenotypes and pretreatment-generated inhibitors tolerance profile of isolate NGY10. a Ploidy determination: Total DNA content of the cells was analyzed by flow cytometry followed by propidium iodide (PI) staining. Ploidy was determined by comparing the FACS spectra of isolate NGY10 with the spectra of reference haploid (CEN.PK-1137D) and diploid (CEN.PK-122) strains. Unstained and stained cells are represented by purple and green colors. b Thermotolerant phenotype: isolate NGY10 cells were grown in YEPD broth at 30 °C (filled black diamonds) and 40 °C (clear black diamonds), respectively, followed by OD600 measurement after every 30 min interval. c Pre-treatment inhibitors-tolerant phenotypes: Serial tenfold dilution of isolate NGY10 cells (OD600 = 0.1) was spotted on SD agar plates containing 2.0% glucose and inhibitors (Furfural 1.5 g/l, 5-HMF 3.0 g/l, acetic acid 0.2% v/v and ethanol 10% v/v), and incubated at 40 °C for 24 h