Fig. 6

Acid- and alkali-pretreated RS fermentation via SSF without pre-saccharification (blue coloured bar) and with pre-saccharification (orange coloured bar), using isolate NGY10. SSF was performed at 40 °C for 72 h employing 15 FPU cellulase/g of RS and 5.0% v/v inoculums of overnight YEPD grown isolate NGY10. a With 5.0% w/v solid loading and b with 10.0% w/v solid loading. For SSF without pre-saccharification both cellulase and inoculums were added simultaneously; however, in case of pre-saccharification, cellulases were added to RS and incubated at 50 °C for 6 h before adding the yeast inoculums