Evaluation of divergent yeast genera for fermentation-associated stresses and identification of a robust sugarcane distillery waste isolate Saccharomyces cerevisiae NGY10 for lignocellulosic ethanol production in SHF and SSF

Pandey, Ajay Kumar; Kumar, Mohit; Kumari, Sonam; Kumari, Priya; Yusuf, Farnaz; Jakeer, Shaik; Naz, Sumera; Chandna, Piyush; Bhatnagar, Ishita; Gaur, Naseem A.

doi:10.1186/s13068-019-1379-x

Biotechnology for Biofuels and Bioproducts

Table 2 Specific growth rate and doubling time of yeast isolates at 30 °C and 40 °C in YEPD medium

From: Evaluation of divergent yeast genera for fermentation-associated stresses and identification of a robust sugarcane distillery waste isolate Saccharomyces cerevisiae NGY10 for lignocellulosic ethanol production in SHF and SSF

S. no.	Yeast strains	30 °C		40 °C		% increase in T_d at 40 °C	% decrease in µ at 40 °C
S. no.	Yeast strains	Doubling time (T_d), (min)	Specific growth rate (µ) (h⁻¹)	Doubling time (T_d) (min)	Specific growth rate (µ) (h⁻¹)	% increase in T_d at 40 °C	% decrease in µ at 40 °C
1.	Angel yeast	75.21 ± 1.63	0.552 ± 0.05	81.63 ± 1.06	0.509 ± 0.05	8.53	7.78
2.	S. cerevisiae CEN-PK-122	91.18 ± 1.06^a	0.456 ± 0.02	100.05 ± 1.7	0.415 ± 0.05	9.72	8.86
3.	S. cerevisiae NCIM 3570	98.07 ± 1.59	0.424 ± 0.03	108.6 ± 1.5	0.383 ± 0.06	10.7	9.67
4.	S. cerevisiae NGY1	85.56 ± 0.53	0.486 ± 0.01	90.98 ± 0.49	0.457 ± 0.04	6.34	5.97
5.	S. cerevisiae NGY10	73.20 ± 1.21	0.568 ± 0.023	76.014 ± 1.13	0.547 ± 0.05	3.84	3.69
6.	K. marxianus NCIM 3465	57.04 ± 1.01	0.729 ± 0.019	59.10 ± 0.93	0.704 ± 0.04	3.55	3.43
7.	K. marxianus NGY8	55.0 ± 1.06	0.756 ± 0.02	56.65 ± 0.98	0.734 ± 0.04	2.99	2.91
8.	K. lactis NCIM 3551	92.4 ± 1.64	0.45 ± 0.031	97.83 ± 1.51	0.425 ± 0.05	5.88	5.56
9.	S. stipitis NCIM 3507	89.04 ± 0.42	0.467 ± 0.008	106.61 ± 0.39	0.39 ± 0.029	19.74	16.49
10.	S. stipitis NCIM 3498	95.15 ± 0.37	0.437 ± 0.007	108.84 ± 0.34	0.382 ± 0.03	14.39	12.58
11.	C. shehatae NCIM 3500	83.83 ± 2.65	0.496 ± 0.05	96.50 ± 2.45	0.431 ± 0.07	15.08	13.10
12.	C. lusitaniae NCIM 3484	89.04 ± 0.95	0.467 ± 0.018	97.61 ± 0.88	0.426 ± 0.04	9.62	8.77
13.	C. albicans SC5314	76.01 ± 1.6	0.547 ± 0.031	82.01 ± 1.52	0.507 ± 0.05	7.9	7.31
14.	W. anomalus NGY2	78.01 ± 1.53	0.533 ± 0.029	84.0 ± 1.42	0.495 ± 0.02	7.68	7.13
15.	O. thermophila NGY11	93.86 ± 1.37	0.443 ± 0.026	98.80 ± 1.3	0.421 ± 0.01	5.23	4.97
16.	C. glabrata CBS138	62.06 ± 1.06	0.67 ± 0.02	67.20 ± 0.98	0.619 ± 0.01	8.24	7.61
17.	C. glabrata NGY7	65.07 ± 3.76	0.639 ± 0.071	68.05 ± 3.47	0.611 ± 0.06	4.58	4.38
18.	C. glabrata NGY14	68.05 ± 2.12	0.611 ± 0.04	72.69 ± 1.96	0.572 ± 0.03	6.81	6.38
19.	P. kudriavzevii NGY12	62.34 ± 2.58	0.667 ± 0.06	68.05 ± 2.94	0.611 ± 0.05	9.16	8.39
20.	P. kudriavzevii NGY13	59.74 ± 1.65	0.696 ± 0.091	62.06 ± 3.00	0.67 ± 0.06	6.86	6.42
21.	P. kudriavzevii NGY15	61.42 ± 1.08	0.677 ± 0.02	65.07 ± 0.98	0.639 ± 0.043	4.85	4.63
22.	P. kudriavzevii NGY16	60.88 ± 2.42	0.683 ± 0.045	66.85 ± 2.21	0.622 ± 0.012	7.72	7.16
23.	P. kudriavzevii NGY20	57.83 ± 2.38	0.719 ± 0.035	59.48 ± 1.72	0.699 ± 0.07	4.85	4.62
24.	C. dubliniensis NGY5	81.05 ± 0.63	0.513 ± 0.012	84.0 ± 0.6	0.495 ± 0.045	3.63	3.51
25.	C. tropicalis NGY3	80.12 ± 1.16	0.519 ± 0.022	83.16 ± 1.1	0.5 ± 0.055	3.8	3.66
26.	C. tropicalis NGY4	84.03 ± 3.28	0.495 ± 0.062	88.47 ± 3.04	0.47 ± 0.095	5.32	5.05
27.	C. tropicalis NGY6	82.33 ± 1.48	0.505 ± 0.028	87.17 ± 1.37	0.477 ± 0.061	5.87	5.54
28.	C. tropicalis NGY9	79.05 ± 1.0	0.526 ± 0.019	84.0 ± 0.93	0.495 ± 0.052	6.26	5.89
29.	C. tropicalis NGY17	80.12 ± 4.3	0.519 ± 0.081	85.03 ± 3.96	0.489 ± 0.052	6.13	5.78
30.	C. tropicalis NGY18	75.05 ± 2.96	0.554 ± 0.056	81.10 ± 2.74	0.513 ± 0.052	7.99	7.4
31.	C. tropicalis NGY19	78.01 ± 3.34	0.533 ± 0.063	84.0 ± 3.08	0.495 ± 0.052	7.67	7.13
32.	C. tropicalis NGY21	84.0 ± 3.13	0.495 ± 0.059	86.81 ± 2.89	0.479 ± 0.052	3.34	3.23
33.	C. tropicalis NGY22	80.11 ± 1.54	0.519 ± 0.029	85.03 ± 1.42	0.489 ± 0.052	6.13	5.78
34.	C. tropicalis NGY23	84.0 ± 4.24	0.495 ± 0.08	90.98 ± 3.92	0.457 ± 0.121	8.32	7.67
35.	C. tropicalis NGY24	81.05 ± 4.87	0.513 ± 0.092	88.09 ± 4.51	0.472 ± 0.133	8.68	7.99
36.	C. tropicalis NGY25	82.33 ± 1.32	0.505 ± 0.025	86.09 ± 1.22	0.483 ± 0.066	4.55	4.36

^aMean ± standard deviation, Experiments were conducted in biological triplicates (n = 3) and value presented in table are their mean values with standard deviation

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