Evaluation of divergent yeast genera for fermentation-associated stresses and identification of a robust sugarcane distillery waste isolate Saccharomyces cerevisiae NGY10 for lignocellulosic ethanol production in SHF and SSF

Pandey, Ajay Kumar; Kumar, Mohit; Kumari, Sonam; Kumari, Priya; Yusuf, Farnaz; Jakeer, Shaik; Naz, Sumera; Chandna, Piyush; Bhatnagar, Ishita; Gaur, Naseem A.

doi:10.1186/s13068-019-1379-x

Biotechnology for Biofuels and Bioproducts

Table 5 Fermentation profile of selected glucose fermenting yeast isolates with acid- and alkali-pretreated rice straw enzymatic hydrolysates at 40 °C in 24 h

From: Evaluation of divergent yeast genera for fermentation-associated stresses and identification of a robust sugarcane distillery waste isolate Saccharomyces cerevisiae NGY10 for lignocellulosic ethanol production in SHF and SSF

S. no.	Yeast strains	Acid pre-treated rice straw enzymatic hydrolysate (APRSEH-1)^b						Alkali pre-treated rice straw enzymatic hydrolysate (APRSEH-2)^c
S. no.	Yeast strains	Ethanol concentration (g/l)	Ethanol yield (g/g)	Ethanol Productivity (g/l//h)	Ethanol efficiency (%)	Glycerol concentration (g/l)	Acetic acid concentration (g/l)	Ethanol concentration (g/l)	Ethanol yield (g/g)	Ethanol productivity (g/l/h)	Ethanol efficiency (%)	Glycerol concentration (g/l)	Acetic acid concentration (g/l)
1.	Angel yeast	11.79 ± 0.08^a	0.451	0.491	88.258	0.47 ± 0.03	0.52 ± 0.02	7.02 ± 0.02	0.456	0.293	89.24	0.46 ± 0.002	0.73 ± 0.02
2.	S. cerevisiae CEN-PK-122	11.65 ± 0.21	0.441	0.485	86.423	1.38 ± 0.07	0.78 ± 0.03	6.95 ± 0.07	0.41	0.289	80.23	1.56 ± 0.06	0.79 ± 0.03
3.	S. cerevisiae NGY1	11.75 ± 0.13	0.445	0.489	87.165	0.6 ± 0.02	0.54 ± 0.02	7.09 ± 0.09	0.454	0.295	88.85	0.39 ± 0.012	0.24 ± 0.01
4.	S. cerevisiae NGY10	12.25 ± 0.09*	0.474	0.51	92.812	0.54 ± 0.02	0.47 ± 0.011	7.18 ± 0.04*	0.468	0.298	91.58	0.33 ± 0.005	0.18 ± 0.002
5.	K. marxianus NCIM 3465	10.89 ± 0.16	0.412	0.453	80.785	0.85 ± 0.04	0.66 ± 0.02	6.19 ± 0.03	0.361	0.257	70.64	0.64 ± 0.021	0.36 ± 0.002
6.	K. marxianus NGY8	11.55 ± 0.08	0.437	0.481	85.681	0.37 ± 0.01	1.87 ± 0.07	6.98 ± 0.07	0.41	0.29	80.23	0.16 ± 0.003	1.57 ± 0.008
7.	C. glabrata CBS138	10.17 ± 0.07	0.419	0.423	82.003	0.28 ± 0.03	0.74 ± 0.032	5.67 ± 0.13	0.331	0.236	64.77	0.07 ± 0.002	0.45 ± 0.01
8.	C. glabrata NGY7	10.92 ± 0.13	0.436	0.455	85.513	0.34 ± 0.02	0.81 ± 0.03	7.02 ± 0.06	0.43	0.292	84.14	0.13 ± 0.003	0.52 ± 0.002
9.	P. kudriavzevii NGY12	9.45 ± 0.16	0.425	0.393	83.264	0.34 ± 0.01	0.67 ± 0.03	6.95 ± 0.11	0.41	0.289	80.23	0.13 ± 0.001	0.39 ± 0.003
11.	P. kudriavzevii NGY20	10.51 ± 0.17	0.456	0.437	89.346	0.34 ± 0.02	0.67 ± 0.033	5.71 ± 0.12	0.332	0.237	64.97	0.13 ± 0.001	0.38 ± 0.002
12.	C. tropicalis NGY19	10.48 ± 0.11	0.464	0.436	90.988	0.47 ± 0.03	0.46 ± 0.021	4.98 ± 0.08	0.29	0.207	56.75	0.26 ± 0.011	0.04 ± 0.001

^aMean ± standard deviation for n = 3; Fermentation pH-5.4; Initial sugar = 26.38 g/l, inoculums 5.0% v/v ≈ 1.0 × 10⁷ cells/ml, fermentation time 24 h
^bAPRSEH-1 consisted of sugar 33.76 g/l (glucose: 26.38 g/l and xylose: 7.38 g/l), furfural: 0.618 g/l, HMF: 0.748 g/l and acetic acid: 1.91 g/l
^cAPRSEH-2 consisted of sugar 22.78 g/l (glucose: 17.15 g/l and xylose: 5.63 g/l), furfural: 0.142 g/l, HMF:0.148 g/l and acetic acid:0.51 g/l
* Statistical student t-test of isolate NGY10 with reference strains CEN-PK-122 and Angel yeast for glucose fermentation of APRSEH-1 and APRSEH-2 was performed, and show significance, i.e. p < 0.05

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