Fig. 1

Global map of central metabolic pathways in C. glutamicum ATCC 13032 and the heterologous mevalonate-based pathway for isopentenol production. The mevalonate-based isopentenol biosynthesis pathway was described in [28]. Broken lines show intermediate pathways omitted in glycolysis and the tricarboxylic acid cycle. The disrupted pathways in this work are shown in grey and indicated with a red “X”. Relevant reactions are represented by the genes encoding their corresponding enzymes. Reactions involved in isopentenol biosynthesis are displayed with red arrows. Abbreviations: atoB, acetyl-CoA acetyltransferase; HMGS, hydroxymethylglutaryl-CoA synthase; HMGR, 3-hydroxy-3-methylglutaryl-CoA reductase; MK, mevalonate kinase; PMD, phosphomevalonate decarboxylase; MEP, methylerythritol-4-phosphate; MVA, mevalonate; G6P, glucose-6-phosphate; F6P, fructose-6-phosphate; DAH, dihydroxyacetone phosphate; G3P, glyceraldehyde-3-phosphate; PEP, phosphoenolpyruvic acid; HMG-CoA, 3-hydroxy-3-methyl-glutaryl-coenzyme A; HS-CoA, coenzyme A; Pi, inorganic phosphate