Fig. 7

An Engineered C. glutamicum Host Chassis With Modified Isopentenol Production Pathway Produces Isopentenol Using IL-Pretreated Sorghum Biomass. a Analysis of the engineered isopentenol production pathway using a hmgr homolog from S. pomeroyi in a 5 mL cultivation format: Isopentenol production was performed in a ∆poxB ∆ldhA strain harboring either the original isopentenol production pathway or a variant where the S. cerevisiae HMGR was replaced with a hmgr homolog from S. pomeroyi. One additional timepoint at 72 h post-induction was harvested. b Isopentenol production using IL-pretreated sorghum hydrolysate: CGXII minimal media was prepared as described previously in Fig. 5a. C. glutamicum ∆poxB ∆ldhA was adapted to growth in CGXII minimal media containing hydrolysate and assessed for isopentenol production at the timepoints indicated. All isopentenol values are the average of biological triplicates, and the error bars represent standard error