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Fig. 3 | Biotechnology for Biofuels

Fig. 3

From: Seed culture pre-adaptation of Bacillus coagulans MA-13 improves lactic acid production in simultaneous saccharification and fermentation

Fig. 3

Time-course of batch SSF performed with B. coagulans MA-13 at a cell/biomass ration of 0.01 gcells/gWIS. SSF experiments were carried out anaerobically in 3.6 L BioEtOH double-jacket flat-bottom vessels at 55 °C and pH 5.5. Pre-hydrolysis of the WIS fraction was carried out for 30 min by adding 10 FPU/gWIS of Cellic CTec 2 before inoculating cells from the seed cultures. The fermentation medium (10% WIS from pre-treated wheat-straw) was inoculated with seed cultures propagated in hydrolysate-free medium (a) or in media containing 30% (b), 40% (c) and 50% (d) hydrolysate. Glucose consumption (blue circle), lactic acid production (green triangle) and total CFU (black circle) are reported. Error bars indicate minimum and maximum values in duplicate experiments

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