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Table 1 Positive molecular ions analysis of HiPMO1 reaction products hydrolyzed by beta-glucuronidase and beta-glucosidase

From: Polysaccharide monooxygenase-catalyzed oxidation of cellulose to glucuronic acid-containing cello-oligosaccharides

m/z Molecular ions of potential products m/z Molecular ions of potential products
102 180 + Na+ + H+/2 181 180 + H+
103 182 + Na+ + H+/2 184 192 + 174 + 2H+/2
106 210 + H+ + H+/2 185 194 + 174 + 2H+/2
120 192 + 2H+ + HCOOH/2 186 196 + 174 + 2H+/2
122 176 + 2NH4+ + CH3OH/2 187 180 + 192 + 2H+/2
123 210 + 2NH4+/2 191 174 + 174 + 2H+ + CH3OH/2
145 194 + 2NH4+ + CH3COOH/2 193 192 + H+
149 180 + 2NH4+ + HCOOH + 2H2O/2 195 194 + H+
194 + 2NH4+ + CH3OH + 2H2O/2 203 180 + Na+
152 180 + 2Na+ + CH3COOH + H2O/2 206 192 + 174 + 2Na+/2
194 + 2Na+ + HCOOH + H2O/2 210 192 + NH4+
153 210 + 2NH4+ + CH3COOH/2 212 194 + NH4+
192 + 2NH4+ + H2O + CH3COOH/2 214 196 + NH4+
158 210 + 2Na+ + CH3COOH/2 215 192 + Na+
192 + 2Na+ + H2O + CH3COOH/2 216 180 + NH4+ + H2O
161 180 + 2Na+ + CH3COOH + 2H2O/2 217 194 + Na+
194 + 2Na+ + HCOOH + 2H2O/2 228 210 + NH4+
163 196 + Na+ + H+ + CH3COOH + HCOOH/2 232 196 + NH4+ + H2O
210 + Na+ + H+ + 2HCOOH/2 233 210 + Na+
166 210 + 2H+ + 2CH3COOH/2 254 176 + NH4+ + CH3COOH
192 + 2H+ + H2O + 2CH3COOH/2 256 178 + NH4+ + CH3COOH
167 196 + 2Na+ + CH3COOH + CH3OH/2 192 + NH4+ + HCOOH
210 + 2Na+ + HCOOH + CH3OH/2 257 196 + H+ + CH3COOH
168 180 + 2NH4+ + 2CH3COOH/2 210 + H+ + HCOOH
194 + 2NH4+ + HCOOH + CH3COOH/2 264 210 + NH4+ + 2H2O
170 180 + 2NH4+ + 2HCOOH + CH3OH/2 265 210 + Na+ + CH3OH
194 + 2NH4+ + HCOOH + 2CH3OH/2 196 + Na+ + HCOOH
173 194 + 2Na+ + CH3COOH + HCOOH/2 267 174 + H+ + 2HCOOH
180 + 2Na+ + 2CH3COOH/2 272 194 + NH4+ + CH3COOH
174 156 + NH4+ 273 194 + H+ + CH3COOH + H2O
175 174 + H+ 274 196 + NH4+ + CH3COOH
178 180 + 174 + 2H+/2 210 + NH4+ + HCOOH
179 178 + H+ 294 180 + NH4+ + CH3COOH + 2H2O
180 180 + 178 + 2H+/2 194 + NH4+ + HCOOH + 2H2O
  1. The products were analyzed by Full Scan LC–MS in positive mode. Data are from Additional file 1: Figure S5. Because of the presence of NH3, CH3OH, HCOOH, and CH3COOH in the sample solutions, the products formed adduct ions with them. Glucose (m/z 180+), gluconic acid (m/z 196+), gluconic acid lactone (m/z 178+), glucuronic acid (m/z 194+), glucuronic acid lactone (m/z 176+), saccharic acid (m/z 210+), saccharic acid lactone (m/z + 192), reduced Vc (m/z 176+), oxidized Vc (m/z 174+), oxidized and dehydrate Vc (m/z 156+)