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Fig. 3 | Biotechnology for Biofuels

Fig. 3

From: Creation of a functional hyperthermostable designer cellulosome

Fig. 3

Thermostability of the chimaeric trivalent scaffoldin (ScafGTV) either alone, or complexed to the different dockerin-bearing Cel5D chimeras. ae Display the relative intensities of the designated bands on non-denaturing PAGE gels after 24 h of incubation at 70 °C, 75 °C and 80 °C, quantified by ImageJ. f An example of the corresponding gel bands of the three chimaeric dockerin-bearing Cel5G complexed to ScafGTV incubated at 75 °C for 0 to 24 h. Denaturation assays were reproduced in triplicate, the data represent the mean ± SD

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