Fig. 5
From: Creation of a functional hyperthermostable designer cellulosome
Comparison of the performance of the hyperthermophilic designer cellulosome to the wild-type systems. The relative activities of the Ca. bescii-based designer cellulosome (red bars), chimaeric Ca. bescii enzymes complexed to their monovalent scaffoldin (dark pink), wild-type uncomplexed Ca. bescii enzymes (light pink) and Cl. thermocellum cellulosome (tan) were determined at desired time intervals. Enzymatic activity is defined as mM of released sugars after 24 to 72 h incubation of the enzymes with 4% of Avicel substrate. Each reaction was conducted in triplicate; the data represent the mean ± SD