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Table 1 Substrate utilization, ABE productivity and yield of recombinant C. beijerinckii (Cb) strains during fermentation of glycerol + glucose medium

From: Metabolic engineering of Clostridium beijerinckii to improve glycerol metabolism and furfural tolerance

Recombinant Cb strain Glycerol utilized Glucose utilized ABE
Conc. (g/L) % Improvement vs. control Conc. (g/L) Rate (g/L/h) Conc. (g/L) X (g/L/h) Y (g/g)
pWUR460_gldA1 21.61 ± 2a 25.2 35.1 ± 2.3 0.96a 13 ± 0.01a 0.22a 0.24a
pWUR459_gldA1 23.1 ± 2.1a 33.8 34.8 ± 4.5 1.24b 12.8 ± 1.2a 0.35b 0.22a
pWUR460_dhaD1 22 ± 0.12a 27.35 35 ± 1.9 1.01a 13.1 ± 0.1a 0.22a 0.23a
pWUR459_dhaD1 23.4 ± 3.1a 35.5 35.1 ± 1.3 1.3b 13 ± 0a 0.22a 0.23a
pWUR460_gldA1 + dhaK 23.67 ± 0a 37.14 35.2 ± 1.2 0.98a 13.2 ± 1.1a 0.22a 0.22a
pWUR459_ gldA1 + dhaK 24.4 ± 0.8a 41.4 35.1 ± 0.8 1.18c 12.8 ± 0.7a 0.36b 0.22a
pWUR460_dhaD1 + gldA1 24.6 ± 0.1a 42.94 35 ± 0.2 1.01a 13.2 ± 0.1a 0.28a 0.22a
pWUR459_[(dhaD1 + gldA1) dhaK] 23.2 ± 0a 28.62 34.8 ± 1.2 1.22a 12.5 ± 0.5a 0.26a 0.22a
pMTL500E P (−) adc P (−) thl (Control) 17.26 ± 1b 34.8 ± 0.8 1.14c 11.3 ± 0.4b 0.18c 0.22a
  1. X productivity; Y yield
  2. a,b,cFisher’s least significant difference (p < 0.1) within parameters for each insert versus the plasmid control. Values with same supesrcript are not significantly different (p  < 0.1)