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Table 2 Substrate utilization, furfural detoxification, cell growth (optical density; OD600), butanol and ABE concentrations (C), ABE yield (Y) and productivity (Z) profiles of C. beijerinckii (Cb) pWUR460_dhaD1 + gldA1 grown in furfural (2 to 6 g/L)-supplemented glucose + glycerol medium

From: Metabolic engineering of Clostridium beijerinckii to improve glycerol metabolism and furfural tolerance

Furfural (g/L) Cb strain Max. cell OD600 Butanol ABE Substrate utilized R (g/L/h)
C (g/L) Y (g/g) Z (g/L/h) C (g/L) Y (g/g) Z (g/L/h) Glucose (g/L) Glycerol (g/L)
2 pWUR460_ dhaD1 + gldA1 5.5 ± 0.1a 8.7a 0.14a 0.18a 12.4 ± 0.1a 0.20a 0.26a 35.6 ± 0.1a 29.1 ± 1.1a 1.36a
Plasmid control 3.5 ± 0.02b 8.7 ± 0.1b 0.14a 0.18a 12.1 ± 1.1a 0.20a 0.20b 35.8 ± 1.1a 28.9 ± 0.9a 1.37a
3 pWUR460_ dhaD1 + gldA1 4.0 ± 0.03a 8.5 ± 0.9a 0.13a 0.18a 12.4 ± 0.5a 0.20a 0.26a 35.8 ± 0.2a 29.1 ± 0.1a 1.36a
Plasmid control 2.8 ± 0.1b 8.2 ± 1.1b 0.15b 0.17a 12.4 ± 0.1a 0.19a 0.20b 36 ± 0.23a 20.1 ± 0.9b 1.4a
4 pWUR460_ dhaD1 + gldA1 3.7 ± 0.2a 6.9 ± 0.2a 0.11a 0.14a 10.1 ± 0.5a 0.16a 0.21a 35.4 ± 0.5a 26.2 ± 1.1a 1.15a
Plasmid control 2.4 ± 0.2b 6.2 ± 0.4b 0.11a 0.13a 9.4 ± 0.34b 0.17a 0.20a 35.7 ± 0.1a 18.6 ± 0.2b 1.13a
5 pWUR460_ dhaD1 + gldA1 3.6 ± 0.2a 5.6a 0.11a 0.12a 8.6 ± 0.7a 0.17a 0.18a 25.4 ± 0.2a 23.9 ± 0.5a 1.38a
Plasmid control 2.1 ± 0.5b 5.1 ± 0.8b 0.12a 0.11a 8.1 ± 0.01b 0.18a 0.17a 31.1 ± 0.1b 13.1 ± 0.1b 0.82b
6 pWUR460_ dhaD1 + gldA1 2.7 ± 0.9a 4.5 ± 0.9a 0.2a 0.09a 7.7a 0.34a 0.16a 9.8 ± 0a 13 ± 0.8a 1.24a
Plasmid control 1.9b 3.1 ± 0.3b 0.16a 0.06b 5.5b 0.28b 0.12b 12 ± 0.1b 7.5 ± 0.23b 0.96b
  1. C concentration, Y yield, X productivity, R rate of furfural detoxification
  2. a,bFisher’s LSD (p < 0.1) between means of Cb pWUR460_ dhaD1 + gldA1 vs. the plasmid control for each furfural concentration within each parameter