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Fig. 3 | Biotechnology for Biofuels

Fig. 3

From: On the functional characterization of lytic polysaccharide monooxygenases (LPMOs)

Fig. 3

Soluble products generated by C4-oxidizing NcLPMOs from PASC or TXG in reactions fueled by O2/Ascorbic acid or H2O2. a, b HPAEC-PAD profiles of products generated in reaction mixtures containing 1 mM ascorbic acid and 1 μM NcLPMO9A (black line), 1 μM NcLPMO9C (red line) or 1 μM NcLPMO9D (blue line) and 2 mg mL−1 of a PASC or b TXG. c, d HPAEC-PAD profiles of products generated in reaction mixtures fueled by H2O2 containing 1 μM NcLPMO9A (black line), 1 μM NcLPMO9C (red line) or 1 μM NcLPMO9D (blue line), and 2 mg mL−1 of c PASC or d TXG. In these latter reactions, ~ 45 μM of H2O2 was added to the reactions every 15 min; prior to every addition of H2O2, ~ 12 μM of ascorbic acid was added to ensure reduction of the LPMO. All the reactions were performed in standard aerobic conditions, i.e., in the presence of approximately 250 μM O2. The labeling of cello-oligosaccharides in a and c is based on previous work [19]. The large variation in retention times between a and c and between b and d is due to the fact that chromatograms were produced at different time points; in between, both columns and parts of the chromatographic system were replaced. These figures are derived from an unpublished study by Petrovic et al., which will be published elsewhere

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