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Fig. 7 | Biotechnology for Biofuels

Fig. 7

From: On the functional characterization of lytic polysaccharide monooxygenases (LPMOs)

Fig. 7

The importance of using progress curves when making quantitative statements on LPMO properties. The graphs show LPMO products generated by a series of engineered variants of a bacterial C1/C4-oxidizing LPMO from Micromonospora aurantiaca called MaLPMO10B. a Product levels after 2 h; b product levels after 24 h. Clearly, if the mutants had been characterized by assessing only one time point, important information would have been missed and the conclusions of the study would have been strongly influenced by the choice of time point. Of note, some of the variants were likely already completely activated at 2 h (e.g., N85F), which implies that their initial catalytic rates may be higher than suggested by the product levels observed after 2 h. The data shown here are from [58]. See [65] for a similar example

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