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Fig. 5 | Biotechnology for Biofuels

Fig. 5

From: Kluyveromyces marxianus developing ethanol tolerance during adaptive evolution with significant improvements of multiple pathways

Fig. 5

RT-qPCR analysis for gene differential expression of KM and KM-100d in different groups. a KM-100d vs. KM both in the ethanol-free medium. This is for DE gene analysis in Group ①. b KM exposed in 4% (v/v) ethanol vs. KM in an ethanol-free medium. This is for Group ⑥. c KM exposed in 6% (v/v) ethanol vs. KM in an ethanol-free medium. This is for Group ⑦. d KM-100d exposed in 4% (v/v) ethanol vs. KM-100d in an ethanol-free medium. This is for Group ④. e KM-100d exposed in 6% (v/v) ethanol vs. KM-100d in an ethanol-free medium. This is for Group ⑤. In each sample, 18S was used as an internal control. Total RNA was isolated from cells cultured at 48 h in biological triplicate

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