Skip to main content
Fig. 4 | Biotechnology for Biofuels

Fig. 4

From: QTL analysis reveals genomic variants linked to high-temperature fermentation performance in the industrial yeast

Fig. 4

Mapping of major (a) and minor (b) thermotolerance-related QTLs by pooled-segregant whole-genome sequence analysis. a In major QTL mapping, the SNP frequencies refer to the percentage of the SNP nucleotide in the pools originating from the thermotolerant parent strain ScY01α-tp. b In minor QTL mapping, the SNP frequencies refer to the percentage of the SNP nucleotide in the pools originating from the thermosensitive parent strain W65a-tp. In the upper panels of a and b, scatter plots of SNP frequency versus chromosome are shown. The raw data of SNP frequencies are shown as dots, smoothened using a Linear Mixed Model [30] and shown in bold lines. Green, red and purple dots and lines represent the raw data and smoothed data of SNP frequencies in superior pool, inferior pool and random pool, respectively. In the bottom panels of a and b, QTL detections using the EXPLoRA and MULTIPPOL methods are shown. The green line represents the probability of linkage obtained by EXPLoRA, where peak regions showed higher SNP frequencies than 0.5 and were, therefore, detected as QTLs. The red line represents LOD scores in superior pool versus inferior pool calculated by MULTIPOOL, whereas the purple line represents LOD scores in superior pool versus random pool. When both maximum LOD scores were higher than 5, this locus was detected as a QTL by MULTIPOOL. QTLs were further narrowed down by analysing whether nonsynonymous amino acid changes were present. Eventually, two major QTLs named QTL1 and QTL2 as well as two minor QTLs named QTL3 and QTL4 were identified

Back to article page