Fig. 2
The schematic illustration of the dual reporter-gene system based on the pEZ15A shutter vector, which includes the reporter gene opmCherry driven by the constitutive promoter PlavUV5 and terminated by rrnB T1 terminator as the intrinsic control, another reporter gene EGFP under the control of the targeted genetic element and terminated by rrnB T1 terminator, and a terminator BBa_B0014 inserted between these two reporter-gene expression cassettes (a); evaluation of the dual reporter-gene system under Ptet inducible system using different approaches of qPCR (b, c), flow cytometry (FCM, d, e) and Western blot (f), as well as the correlations of FCM results with qPCR and Western blot results, respectively (g). The concentrations of tetracycline are 0, 0.2, 0.4, 0.6, 0.8, or 1.0 μg/mL, respectively