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Fig. 1 | Biotechnology for Biofuels

Fig. 1

From: CRISPR–Cas ribonucleoprotein mediated homology-directed repair for efficient targeted genome editing in microalgae Nannochloropsis oceanica IMET1

Fig. 1

Approach; a the purified Cas protein is assembled with guide RNA to form the active RNP complex which is transformed along with the editing template into the competent Nannochloropsis oceanica IMET1 cells. The transformed cells are selected on ASW-NB plated with zeocin and ammonia and the mutants are screened by cPCR. b The Cas RNP introduces DSB at the target site which is repaired by HDR in presence of the editing template resulting in targeted mutants

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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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