Fig. 1

Cell growth of the acetate sensitive knockouts. K. phaffii phenotypes of wild-type GS115 (WT), ΔPAS_chr3_1091 (ΔHRK1), ΔPAS_chr4_0402, and ΔPAS_chr4_0812 are shown. Cells were spotted on YPD agar plates (pH 4.5) with acetate of 0, 30 and 40 mM at three concentrations (OD₆₀₀ = 0.1, 0.01 and 0.001). These strains were selected from among the 92 kinase gene knockouts (Additional file 1: Fig. S2)