Fig. 4

Introducing acetate transport gene ScFPS1* for 6-MSA biosynthesis. Cell growth (a), 6-MSA production (b) and 6-MSA productivity (c) of recombinant strains XN and XN-ScFPS1* in medium with acetate of 0, 20 and 30 mM, respectively. Culture conditions were as same as that described in Fig. 3. Acetate concentrations were marked on top of the figure. Statistical significance of 6-MSA production and productivity by overexpression of ScFPS1* relative to the parent strain of XN at each time point is also shown. **P < 0.01, *P < 0.05 at 24 h; ^##P < 0.01 at 48 h; ^⁜⁜P < 0.01, ^⁜P < 0.05 at 72 h; N.S., not significance. n = 6 for each strain at specific time points. The error bars represent the standard deviation of three biological replicates assayed in duplicate