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Fig. 5 | Biotechnology for Biofuels

Fig. 5

From: Modulation of acetate utilization in Komagataella phaffii by metabolic engineering of tolerance and metabolism

Fig. 5

Overexpression of yeast acetyl-CoA synthetase and E. coli acetate kinase/phosphotransacetylase gene for 6-MSA biosynthesis. Cell growth (a), 6-MSA production (b) and 6-MSA productivity (c) of recombinant strains XN, XN-PpACS1, XN-ScACS1* and XN-pta/ackA in medium with acetate of 0, 20 and 30 mM, respectively. Culture conditions were as same as that described in Fig. 3. Acetate concentrations were marked on top of the figure. Acetate concentrations were marked the same as those in Fig. 3. Statistical significance of 6-MSA production and productivity by overexpression of PpACS1, ScACS1* and pta/ackA relative to the parent strain of XN at each time point is also shown. **P < 0.01, *P < 0.05 at 24 h; ##P < 0.01, #P < 0.05 at 48 h; ⁜⁜P < 0.01, P < 0.05 at 72 h; N.S., not significance. n = 6 for each strain at specific time points. The error bars represent the standard deviation of three biological replicates assayed in duplicate. PpAcs1, acetyl-CoA synthetase from K. phaffii. ScAcs1*, acetyl-CoA synthetase from S. cerevisiae. AckA, E. coli acetate kinase. Pta, E. coli phosphotransacetylase

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