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Fig. 3 | Biotechnology for Biofuels

Fig. 3

From: Identification of functional butanol-tolerant genes from Escherichia coli mutants derived from error-prone PCR-based whole-genome shuffling

Fig. 3

Evaluation of butanol tolerance stability and high-concentration butanol tolerance of BW1847 and BW1857. Strains were cultured in LB containing 0 (a), 0.75 (b), 1.0 (c), 1.25 (d), or 1.5% (e) (v/v) butanol. Solid and hollow symbols indicate the strains subjected or not, to 50 generations of subculture, respectively. Rectangles, circles, and triangles represent the initial strains BW25113, BW1847, and BW1857, respectively. f Growth of strains cultured in 1.6–2.0% butanol

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