Fig. 5

Growth curve and butanol efflux of site-specific mutants. The mutant DT385 with mutated acrB_C1198T and DT900 with a truncated rob_AT686–7 gene were obtained by genome-wide site-specific methods. The two strains and their gene-deleted mutants D385 and D900 were cultured in LB with (b, d) or without (a, c) 0.75% butanol. The left and right axes indicate the butanol concentration in media and the cell density (OD₆₀₀), respectively