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Table 1 Mutations identified in the BW1847 and BW1857 strains

From: Identification of functional butanol-tolerant genes from Escherichia coli mutants derived from error-prone PCR-based whole-genome shuffling

Strains

Gene/LC

Function

Mutationa

Gene position/length

Genome position

Effectb

BW1847 and BW1857

cdsA/RS00875

Phosphatidate cytidylyltransferase

G/T

751/858

192,914

G (251) C

pgsA/RS10005

CDP-diacylglycerol-glycerol-3-phosphate 3-phosphatidyltransferase

T/G

446/594

1,985,853

V (149) G

yheQ/RS11070

Hypothetical protein

T/G

1843/1845

2,204,421

E (615)c

hycD/RS14165

Formate hydrogenlyase subunit

C/A

899/1572

2,839,873

A (300) E

aslB/RS19735

Anaerobic sulfatase maturase AslB

T/A

1223/1538

3,977,540

V (408) E

RS08320–RS08395

   

1,658,639–1,672,922

14-kb deletion

BW1847

acrB/RS02385

Multidrug efflux RND transporter permease subunit AcrB

C/T

1198/3792

478,662

L (400) F

spoT/RS18950

Bifunctional (p)ppGpp synthetase II/guanosine-3′,5′-bis pyrophosphate 3′-pyrophosphohydrolase

G/T

1882/2109

3,817,641

E (628)c

nusG/RS20660

nusG transcription termination/antitermination

G/T

436/546

4,168,106

G (146) C

rob/RS22900

Right oriC-binding transcriptional activator, AraC family

AT/

1379

4,624,441 (2)

Deletionc

BW1857

rplB/RS17195

50S ribosomal protein L2

T/A

491/822

3,444,233

I (164) N

infB/RS16425

Translation initiation factor IF-2

 

331–555/2673

3,308,845–3,309,069

225 bp deletionc

  1. LC locus number
  2. aThe nucleotide on the left of the backslash was deleted in the corresponding gene, and that on the right of the backslash was inserted in the corresponding gene
  3. bAmino acid position is in parentheses, amino acid residue on the left and right side of parentheses denotes the original and that substituted in the corresponding protein, respectively
  4. cTermination codon