Table 2 Strains used in functional complementary study
Strains | Description | Sources |
|---|---|---|
E. coli Top10F′ | Host for plasmid construction | Lab stock |
E. coli BW25113 | Host for plasmid expression, F− Δ(araD-araB)567 ΔlacZ4787(::rrnB-3)λ−rph-1 Δ(rhaD-rhaB)568 hsdR514 | Lab stock |
BW25113 (pBAD30) | pBAD30 is a arabinose-inducible expression vector, with ori 15A replicon, ApR | This study |
BW25113 (pW/M005) | BW25113 (pBAD30 carries wild/mutated pgsA) | This study |
BW25113 (pW/M070) | BW25113 (pBAD30 carries wild/mutated yehQ) | This study |
BW25113 (pW/M165) | BW25113 (pBAD30 carries wild/mutated hycD) | This study |
BW25113 (pW/M735) | BW25113 (pBAD30 carries wild/mutated aslB) | This study |
BW25113 (pW/M875) | BW25113 (pBAD30 carries wild/mutated cdsA) | This study |
BW25113 (pW/M385) | BW25113 (pBAD30 carries wild/mutated acrB) | This study |
BW25113 (pW/M660) | BW25113 (pBAD30 carries wild/mutated nusG) | This study |
BW25113 (pW/M900) | BW25113 (pBAD30 carries wild/mutated rob) | This study |
BW25113 (pW/M950) | BW25113 (pBAD30 carries wild/mutated spoT) | This study |
BW25113 (pW/M425) | BW25113 (pBAD30 carries wild/mutated infB) | This study |
BW25113 (pW/M195) | BW25113 (pBAD30 carries wild/mutated rplB) | This study |
D005 (W/M005) | BW25113 ΔpgsA::cmr (pBAD30 carries wild/mutated pgsA) | This study |
D070 (pW/M070) | BW25113 ΔyehQ::cmr (pBAD30 carries wild/mutated yehQ) | This study |
D165 (pW/M165) | BW25113 ΔhycD::cmr (pBAD30 carries wild/mutated hycD) | This study |
D735 (pW/M735) | BW25113 ΔaslB::cmr (pBAD30 carries wild/mutated aslB) | This study |
D875 (pW/M875) | BW25113, ΔcdsA::cmr (pBAD30 carries wild/mutated cdsA) | This study |
D385 (pW/M385) | BW25113, ΔacrB::cmr (pBAD30 carries wild/mutated acrB) | This study |
D660 (pW/M660) | BW25113, ΔnusG::cmr (pBAD30 carries wild/mutated nusG) | This study |
D900 (pW/M900) | BW25113, Δrob::cmr (pBAD30 carries wild/mutated rob) | This study |
D950 (pW/M950) | BW25113, ΔspoT::cmr (pBAD30 carries wild/mutated spoT) | This study |
D425 (pW/M425) | BW25113, ΔinfB::cmr (pBAD30 carries wild/mutated infB) | This study |