| Experimental group | Control group | Incubation time | Temperature |
---|---|---|---|---|
Experimental design | 2 mL crude enzyme + 98 mL PH 5.6 PBS buffer + 10 μL tetracycline + 5 g BSPs | 100 ml PH 5.6 PBS buffer + 10 μL tetracycline + 5 g BSPs | 6 days | 37 °C |
Determination index and methods | Low-molecular weight products | Lignocellulose | Surface structure of BSPs | Reducing sugar |
GC–MS (Raj et al. 2007c) | Van Soest method (1991) | SEM | DNS (Miller et al. 1959) |