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Table 2 Design and determination methods of crude enzyme degradation of bamboo shoot particles (BSPs) in vitro

From: Degradation of bamboo lignocellulose by bamboo snout beetle Cyrtotrachelus buqueti in vivo and vitro: efficiency and mechanism

 

Experimental group

Control group

Incubation time

Temperature

Experimental design

2 mL crude enzyme + 98 mL PH 5.6 PBS buffer + 10 μL tetracycline + 5 g BSPs

100 ml PH 5.6 PBS buffer + 10 μL tetracycline + 5 g BSPs

6 days

37 °C

Determination index and methods

Low-molecular weight products

Lignocellulose

Surface structure of BSPs

Reducing sugar

GC–MS (Raj et al. 2007c)

Van Soest method (1991)

SEM

DNS (Miller et al. 1959)