Fig. 2

Activity and substrate specificity of 10 representative ADOs. a The amounts of hydrocarbons produced in E. coli coexpressing ADO and 7942AAR. The amounts of pentadecane (C15:0), heptadecene (C17:1), and heptadecane (C17:0) and their combined total amount are shown in red, green, blue, and gray, respectively. The values relative to the total amount of hydrocarbon produced in E. coli coexpressing 73102ADO and 7942AAR are shown. b The hydrocarbon-producing activity of ADO relative to that of 73102ADO. The values are expressed as the amount of hydrocarbon divided by the amount of the soluble form of ADO. c Fractions of pentadecane, heptadecene, and heptadecane relative to the total amount of hydrocarbon produced in E. coli. d The substrate specificity of ADO relative to that of 73102ADO. In all panels, the data are shown in descending order of activity. A horizontal dotted line shows the value for 73102ADO. The measurements were carried out more than three times, and the means ± standard errors are shown