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Table 1 Strains and plasmids that were used in this study

From: Modular systems metabolic engineering enables balancing of relevant pathways for l-histidine production with Corynebacterium glutamicum

Strain or plasmid Relevant characteristic(s) Source or references
Strains
 E. coli DH5α F-ϕ80lacZΔM15 Δ(lacZYA-argF)U169 endA1 recA1 hsdR17 (r K m +K ) supE44 thi-1 gyrA96 relA1 phoA Hanahan [31]
 E. coli K-12 MG1655 Wild-type strain DSM 18039; F-, λ-, ilvG-, rfb-50, rph-1 German collection of microorganisms and cell cultures
 C. glutamicum WT Wild-type strain ATCC 13032 American type culture collection
 C. jeikeum K411 Wild-type strain Tauch et al. [84]
 C. glutamicum HIS1 C. glutamicum WT with the feedback inhibition released variant HisGG233H−T235Q (corresponding nucleotide exchanges: ggc742cat, acg748cag) This work
 C. glutamicum HIS2 C. glutamicum HIS1 with additional implementation of Ptuf in front of the operon hisDhisChisBcg2302cg2301 This work
 C. glutamicum HIS3 C. glutamicum HIS2 with additional implementation of Ptuf in front of the operon hisHhisAimpAhisFhisIcg2294 This work
 C. glutamicum HIS4 C. glutamicum HIS3 with additional implementation of Ptuf in front of the operon cg0911hisN This work
 C. glutamicum HIS5 C. glutamicum HIS1 with additional implementation of PdapA–A16 in front of the operon hisEhisG, additional exchange of the translational start codon from the native GTG to ATG for hisE This work
 C. glutamicum HIS6 C. glutamicum HIS4 with additional implementation of PdapA–A16 in front of the operon hisEhisG, additional exchange of the translational start codon from the native GTG to ATG for hisE This work
 C. glutamicum HIS7 C. glutamicum HIS6 with additional implementation of PsodA in front of gene hisF This work
 C. glutamicum HIS8 C. glutamicum HIS7 containing pJC4 purA purB This work
 C. glutamicum HIS9 C. glutamicum HIS8 containing pEC-XT99A_gcv_OP1-Cjk This work
 C. glutamicum HIS10 C. glutamicum HIS9 with additional exchange of the translational start codon from ATG to GTG This work
Plasmids
 pK19mobsacB Kmr, mobilizable (oriT), oriV Schäfer et al. [75]
 pK18mobsacB PaceE dapA-A16 pK18mobsacB carrying the dapA-A16 promoter Buchholz et al. [16]
 pK19mobsacB hisGFB pK19mobsacB carrying the nucleotide sequence of a modified hisG variant that encodes HisG with amino acid exchanges G233H and T235Q (corresponding nucleotide exchanges: ggc742cat, acg748cag) This work
 pK19mobsacB hisD-Ptuf pK19mobsacB carrying promoter exchange to Ptuf for operon hisDhisChisBcg2302cg2301 This work
 pK19mobsacB hisH-Ptuf pK19mobsacB carrying promoter exchange to Ptuf for operon hisHhisAimpAhisFhisI This work
 pK19mobsacB hisN-Ptuf pK19mobsacB carrying promoter exchange to Ptuf for operon cg0911hisN This work
 pK19mobsacB hisE-PdapA–A16 pK19mobsacB carrying promoter exchange to PdapAA16 for operon hisEhisG and an exchange of the translational start codon from the native GTG to ATG for hisE This work
 pJC4 Kmr Cordes et al. [20]
 pJC4 purA purB pJC4 carrying genes purA and purB from C. glutamicum ATCC 13032 under control of Ptuf and TrrnB This work
 pEC-XT99A IPTG-inducible overexpression plasmid Kirchner and Tauch [51]
 pEC-XT99A_gcv_OP1-Cjk IPTG-inducible overexpression plasmid for genes gcvP, gcvT, gcvH, lipA, and lipB from C. jeikeium xxx (A. Hüser, Evonik Nutrition & Care GmbH)