Table 1 Strains and plasmids that were used in this study
Strain or plasmid | Relevant characteristic(s) | Source or references |
|---|---|---|
Strains | ||
 E. coli DH5α | F-ϕ80lacZΔM15 Δ(lacZYA-argF)U169 endA1 recA1 hsdR17 (r −K m +K ) supE44 thi-1 gyrA96 relA1 phoA | Hanahan [31] |
 E. coli K-12 MG1655 | Wild-type strain DSM 18039; F-, λ-, ilvG-, rfb-50, rph-1 | German collection of microorganisms and cell cultures |
 C. glutamicum WT | Wild-type strain ATCC 13032 | American type culture collection |
 C. jeikeum K411 | Wild-type strain | Tauch et al. [84] |
 C. glutamicum HIS1 | C. glutamicum WT with the feedback inhibition released variant HisGG233H−T235Q (corresponding nucleotide exchanges: ggc742cat, acg748cag) | This work |
 C. glutamicum HIS2 | C. glutamicum HIS1 with additional implementation of Ptuf in front of the operon hisD–hisC–hisB–cg2302–cg2301 | This work |
 C. glutamicum HIS3 | C. glutamicum HIS2 with additional implementation of Ptuf in front of the operon hisH–hisA–impA–hisF–hisI–cg2294 | This work |
 C. glutamicum HIS4 | C. glutamicum HIS3 with additional implementation of Ptuf in front of the operon cg0911–hisN | This work |
 C. glutamicum HIS5 | C. glutamicum HIS1 with additional implementation of PdapA–A16 in front of the operon hisE–hisG, additional exchange of the translational start codon from the native GTG to ATG for hisE | This work |
 C. glutamicum HIS6 | C. glutamicum HIS4 with additional implementation of PdapA–A16 in front of the operon hisE–hisG, additional exchange of the translational start codon from the native GTG to ATG for hisE | This work |
 C. glutamicum HIS7 | C. glutamicum HIS6 with additional implementation of PsodA in front of gene hisF | This work |
 C. glutamicum HIS8 | C. glutamicum HIS7 containing pJC4 purA purB | This work |
 C. glutamicum HIS9 | C. glutamicum HIS8 containing pEC-XT99A_gcv_OP1-Cjk | This work |
 C. glutamicum HIS10 | C. glutamicum HIS9 with additional exchange of the translational start codon from ATG to GTG | This work |
Plasmids | ||
 pK19mobsacB | Kmr, mobilizable (oriT), oriV | Schäfer et al. [75] |
 pK18mobsacB PaceE dapA-A16 | pK18mobsacB carrying the dapA-A16 promoter | Buchholz et al. [16] |
 pK19mobsacB hisGFB | pK19mobsacB carrying the nucleotide sequence of a modified hisG variant that encodes HisG with amino acid exchanges G233H and T235Q (corresponding nucleotide exchanges: ggc742cat, acg748cag) | This work |
 pK19mobsacB hisD-Ptuf | pK19mobsacB carrying promoter exchange to Ptuf for operon hisD–hisC–hisB–cg2302–cg2301 | This work |
 pK19mobsacB hisH-Ptuf | pK19mobsacB carrying promoter exchange to Ptuf for operon hisH–hisA–impA–hisF–hisI– | This work |
 pK19mobsacB hisN-Ptuf | pK19mobsacB carrying promoter exchange to Ptuf for operon cg0911–hisN | This work |
 pK19mobsacB hisE-PdapA–A16 | pK19mobsacB carrying promoter exchange to PdapA–A16 for operon hisE–hisG and an exchange of the translational start codon from the native GTG to ATG for hisE | This work |
 pJC4 | Kmr | Cordes et al. [20] |
 pJC4 purA purB | pJC4 carrying genes purA and purB from C. glutamicum ATCC 13032 under control of Ptuf and TrrnB | This work |
 pEC-XT99A | IPTG-inducible overexpression plasmid | Kirchner and Tauch [51] |
 pEC-XT99A_gcv_OP1-Cjk | IPTG-inducible overexpression plasmid for genes gcvP, gcvT, gcvH, lipA, and lipB from C. jeikeium xxx | (A. Hüser, Evonik Nutrition & Care GmbH) |