Bamboo lignocellulose degradation by gut symbiotic microbiota of the bamboo snout beetle Cyrtotrachelus buqueti

Table 4 Design and determination methods of gut symbiotic microbiome degradation of bamboo shoot particles (BSPs) in vitro

	Experimental group	Control group	Incubation time	Temperature
Experimental design	2 mL culture + 98 mL pH 7.2 liquid medium 2^a + 5 g BSPs	100 mL pH 7.2 liquid medium 2^a + 5 g BSPs	6 days	37 °C

	Low-molecular-weight products	Lignocellulose	Surface structure of BSPs	Reducing sugar
Determination index and methods	GC–MS [17]	Van Soest method [38]	SEM	DNS [37]

^aA liquid medium (pH 7.2) composed of 0.5-g yeast extract, 0.5-g malt extract, 0.5-g tryptone, 0.5-g NaCl, 0.2-g KH₂PO₄, 0.13-g MgSO₄·7H₂O and 0.5-g CaCl₂

ISSN: 2731-3654