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Table 4 Design and determination methods of gut symbiotic microbiome degradation of bamboo shoot particles (BSPs) in vitro

From: Bamboo lignocellulose degradation by gut symbiotic microbiota of the bamboo snout beetle Cyrtotrachelus buqueti

  Experimental group Control group Incubation time Temperature
Experimental design 2 mL culture + 98 mL pH 7.2 liquid medium 2a + 5 g BSPs 100 mL pH 7.2 liquid medium 2a + 5 g BSPs 6 days 37 °C
  Low-molecular-weight products Lignocellulose Surface structure of BSPs Reducing sugar
Determination index and methods GC–MS [17] Van Soest method [38] SEM DNS [37]
  1. aA liquid medium (pH 7.2) composed of 0.5-g yeast extract, 0.5-g malt extract, 0.5-g tryptone, 0.5-g NaCl, 0.2-g KH2PO4, 0.13-g MgSO4·7H2O and 0.5-g CaCl2