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Table 3 The effects of XX, A3X, XXX, A2XX, and A2+3XX on the \(K_{m}^{\text{app}}\) and Ki values of Cel7A using pNPC as substrate

From: Insight into the role of α-arabinofuranosidase in biomass hydrolysis: cellulose digestibility and inhibition by xylooligomers

Inhibitors

Concentrations (mM)

Km or \(K_{m}^{\text{app}}\) (mM)

Ki (mM)

None

0

0.58

 

XX

1.0

1.01

0.98 ± 0.22

 

0.5

0.87

 
 

0.1

0.68

 

A3X

1.0

0.94

1.62 ± 0.18

 

0.5

0.80

 
 

0.1

0.61

 

XXX

1.0

0.73

3.22 ± 0.32

 

0.5

0.68

 
 

0.1

0.60

 

A2XX

1.0

0.70

4.51 ± 0.85

 

0.5

0.68

 
 

0.1

0.59

 

A2+3XX

1.0

0.66

7.57 ± 1.12

 

0.5

0.61

 
 

0.1

0.59

 
  1. The value of Km was obtained with the reaction without inhibitors