Biochemical characterization of three new α-olefin-producing P450 fatty acid decarboxylases with a halophilic property

Table 2 Steady-state kinetic parameters and reaction turnover numbers (TONs) of the four OleT enzymes

Enzymes	Kinetic parameters			TON^a	TON^b
Enzymes	k_cat (min⁻¹)	K_m (μM)	k_cat/K_m (min⁻¹ μM⁻¹)	TON^a	TON^b
OleT_JE	860 ± 28	41 ± 4	21	95	185
OleT_JH	910 ± 113	46 ± 15	20	100	180
OleT_SQ	570 ± 55	35 ± 10	16	53	144
OleT_SA	600 ± 13	37 ± 7	16	38	94

^aH₂O₂-batch: 1 mM H₂O₂, 1 mM substrate (lauric acid), 1 μM purified OleT, 30 °C, 12 h
^bH₂O₂ fed-batch: 5 times H₂O₂ addition (5 × 200 μM) every 2 h, 1 mM substrate (lauric acid), 1 μM purified OleT, 30 °C, 12 h; mean values and standard errors from two parallel experiments

ISSN: 2731-3654