Enzymes | Relative activities, % |
---|
0 Mc | 0.5 M | 2 M | Saturatedd |
---|
OleT
aJE
| 84 ± 5 | 104 ± 2 | 97 ± 3 | 75 ± 2 |
OleT
aJH
| 77 ± 3 | 102 | 102 | 79 ± 11 |
OleT
aSQ
| 61 ± 3 | 93 ± 2 | 65 ± 6 | 30 ± 6 |
OleT
aSA
| 57 ± 5 | 98 ± 4 | 85 ± 3 | 56 ± 4 |
OleT
bJE
| 74 ± 2 | 95 ± 3 | 83 ± 2 | 67 ± 6 |
OleT
bJH
| 51 ± 6 | 100 ± 6 | 90 ± 1 | 62 ± 8 |
OleT
bSQ
| 56 ± 6 | 112 ± 3 | 68 ± 4 | 21 ± 1 |
OleT
bSA
| 39 ± 10 | 91 ± 23 | 90 ± 3 | 66 ± 12 |
- aRelative % conversion of C12 in comparison to the conversion ratio in the standard reaction buffer without pretreatment
- bRelative % of C11 1-alkene production in comparison to the conversion ratio in the standard reaction buffer without pretreatment
- c“0” means that 1–3 μL OleT enzymes (in storage buffer) were used in a 200 μL NaCl-free reaction buffer
- d“Saturated” means an at least 5 M NaCl concentration