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Table 3 Relative activities of the OleT enzymes after a 12-h treatment at 30 °C in storage buffer with different NaCl concentrations

From: Biochemical characterization of three new α-olefin-producing P450 fatty acid decarboxylases with a halophilic property

Enzymes

Relative activities, %

0 Mc

0.5 M

2 M

Saturatedd

OleT aJE

84 ± 5

104 ± 2

97 ± 3

75 ± 2

OleT aJH

77 ± 3

102

102

79 ± 11

OleT aSQ

61 ± 3

93 ± 2

65 ± 6

30 ± 6

OleT aSA

57 ± 5

98 ± 4

85 ± 3

56 ± 4

OleT bJE

74 ± 2

95 ± 3

83 ± 2

67 ± 6

OleT bJH

51 ± 6

100 ± 6

90 ± 1

62 ± 8

OleT bSQ

56 ± 6

112 ± 3

68 ± 4

21 ± 1

OleT bSA

39 ± 10

91 ± 23

90 ± 3

66 ± 12

  1. aRelative % conversion of C12 in comparison to the conversion ratio in the standard reaction buffer without pretreatment
  2. bRelative % of C11 1-alkene production in comparison to the conversion ratio in the standard reaction buffer without pretreatment
  3. c“0” means that 1–3 μL OleT enzymes (in storage buffer) were used in a 200 μL NaCl-free reaction buffer
  4. d“Saturated” means an at least 5 M NaCl concentration