Assessing the stability and techno-economic implications for wet storage of harvested microalgae to manage seasonal variability

Table 3 Carbohydrate composition of Scenedesmus acutus biomass after 30 or 180 days of wet anaerobic storage

Experimental condition	Length of storage (days)	Glucan (%, db)	Galactan (%, db)	Mannan (%, db)
S. acutus—t₀ ¹	0	26.9 ± 0.04^a	3.7 ± 0.3^a	8.1 ± 0.1^a
Anaerobic*	30	22.8 ± 1.1^b	1.9 ± 0.2^b	8.3 ± 0.1^ab
Anaerobic*	180	23.5 ± 0.7^b	1.3 ± 0.05^b	8.7 ± 0.2^b
Sulfuric acid (0.5%, db)*	30	19.2 ± 0.01^c	1.3 ± 0.1^b	8.4 ± 0.05^ab
Sulfuric acid (0.5%, db)*	180	19.9 ± 0.04^c	1.3 ± 0.04^b	8.2 ± 0.01^a
p value		< 0.001	< 0.001	0.018
S. acutus—t₀ ²	0	21.9 ± 0.4^a	3.1 ± 0.1^a	8.7 ± 0.3^a
Glycosidase*	30	15.2 ± 0.3^b	0.9 ± 0.01^b	8.7 ± 0.01^a
Glycosidase*	180	10.7 ± 0.01^c	1.0 ± 0.00^b	7.1 ± 0.02^b
Glucose oxidase*	30	12.5 ± 0.2^d	1.3 ± 0.1^b	8.1 ± 0.2^a
Glucose oxidase*	180	5.7 ± 0.2^e	1.2 ± 0.0^b	7.0 ± 0.3^b
p value		< 0.001	< 0.001	< 0.001

* Biological replicates (n = 3) were combined to accommodate all analyses. Variation is represented by standard deviation or analytical replicates (n = 2). Letters represent statistically distinct values as determined by Tukey’s test
^1,2Harvest 1 and 2

ISSN: 2731-3654