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Fig. 2 | Biotechnology for Biofuels

Fig. 2

From: Mutation of 4-coumarate: coenzyme A ligase 1 gene affects lignin biosynthesis and increases the cell wall digestibility in maize brown midrib5 mutants

Fig. 2

Identification of the bm5 gene. a The SNP-index for the F2 progeny of bm5-504J. b Genomic location of Zm4CL1 (GRMZM2G075333) on maize chromosome 5. The base pair number was referred to the Phytozome database version 1.8. c Exon and intron structure of the Zm4CL1 gene. Black boxes indicate exons and lines between the boxes indicate introns. Insertion sequences of bm5 are indicated by inverse triangles. d S1 PCR products amplified from B73, bm5-504I, and bm5-505J genomic DNA and S2 PCR products amplified from B73 and bm5-504J genomic DNA. e S1 PCR products amplified from B73, bm5-504I, and bm5-504J cDNA. f RT-PCR analysis of Zm4CL1 expression in midribs of B73 wild-type and bm5 mutants. g qRT-PCR analysis of Zm4CL1 expression in midribs of B73 wild-type and bm5 mutants. The primer pair was designed in 3′ untranslated region. Midribs of the fourth leaves were collected from 60-day-old B73 wild-type and bm5 mutants

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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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