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Fig. 3 | Biotechnology for Biofuels

Fig. 3

From: Mutation of 4-coumarate: coenzyme A ligase 1 gene affects lignin biosynthesis and increases the cell wall digestibility in maize brown midrib5 mutants

Fig. 3

The 4CL activity of putative versions of Zm4CL1 isolated from bm5-504J mutant. a Two domains of Zm4CL1 were labeled. Image was generated using open-source SMART ( b SDS-PAGE analysis of recombinant Zm4CL1, Zm4CL1-L, and Zm4CL1-S proteins. S and I indicated soluble and insoluble proteins from lysate of E. coli cultures. The arrows showed the recombinant proteins. c HPLC analysis of recombinant Zm4CL1 activity against ferulate. d HPLC analysis of recombinant Zm4CL1-L activity against ferulate

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