Table 6 The enzyme hydrolysis of pretreated poplar by different methods

Transgenic poplar

[C₂C₁Im][OAc] at 160 °C for 3 h

10 mg protein of cellulases/g DM in 72 h

< 35.0

[6]

[Ch][Lys] at 140 °C for 1 h

10 mg protein of cellulases/g DM in 72 h

< 40.0

[TBA][OH] at 70 °C for 3 h

10 mg protein of cellulases/g DM in 72 h

< 30.0

Poplar

Steam explosion at 210 °C for 4 min

15 FPU cellulases/g DM in 72 h

< 63.0

[7]

Sacrau poplar

2% NaOH with 1% H₂O₂ at 160 °C for 2 h

20 FPU cellulases/g DM and Tween 80 in 72 h

88.2

[12]

Poplar

6.5% acetic acid at 172 °C for 27 min

20 FPU cellulases/g cellulose in 108 h

< 51.0

[15]

Poplar

0.1 M H₂SO₄ at 160 °C for 35 min

112.5 mg protein of cellulases + 37.5 mg protein of xylanases/g glucan + xylan in 72 h

< 60

[48]

Poplar

Hot water at 180 °C for 12 min

15 mg protein of cellulases/g glucan in 120 h

60.0

[49]

Hybrid poplar

First step: 8% NaOH at 90 °C for 10 h

Second step: 4% Na₂SO₃ at 180 °C for 60 min

15 FPU cellulases/g DM in 72 h

94.5

[5]

Poplar

First step: 0.7% H₂SO₄ at room temperature overnight

Second step: steam explosion at 190 °C for 10 min

32 mg cellulases protein/g DM in 72 h

70

[9]

Poplar

First step: SO₂ steam explosion at 170 °C for 15 min

Second step: ethanol with 1% H₂SO₄ at 170 °C for 60 min

5 FPU cellulases/g cellulose in 72 h

85

[10]

Poplar

First step: 5% AC 170 °C for 30 min

Second step: 80% HPAC at 60 °C for 2 h

7.2 mg protein of cellulases/g DM and Tween 80 in 72 h

86.6

This study

Poplar

First step: 5% AC 170 °C for 30 min

Second step: 80% HPAC at 60 °C for 2 h

14.3 mg protein of cellulases/g DM and Tween 80 in 72 h

93.8

This study