Fig. 2

Clustering of 8 miscanthus genotypes, based on the cell wall traits from their leaves (L) and stems (S), harvested at peak biomass (PB) and senesced (SS) stages. For a given dendrogram, the colour intensities within each row of the adjacent heatmap, represent relative abundances of corresponding cell wall traits in that genotype. Shades of red represent saccharification efficiency indices: the percentages of total glucose (GlcE), xylose (XylE) and arabinose (AraE) released upon enzymatic saccharification. Cell wall compositional features are represented in shades of blue. Ara/Xyl, is the ratio of arabinose to xylose determined in the cell wall material (CWM). N designates the normalised value representing the total epitope abundance (OD/g of CWM) for specific mAbs: xyloglucans (CCRC-M87, galactosylated xyloglucan), glucan (BG1, MLG), xylans (CCRC-M137; CCRC-M144; CCRC-M154) and pectins (CCRC-M7, RG-I/AG; CCRC-M38, homogalacturonan backbone). Only cell wall composition data (blue), and not the three saccharification-related variables (red), were considered for genotype classification, which was performed via agglomerative hierarchical clustering, using the ‘hclust’ R-function. More detail regarding the glycan-directed mAb classes is available in Additional file 1. Genotype sin13 often showed compositional traits misaligned with the remaining M. sinensis genotypes, as its biomass was typically made up of softer tissues than those of other genotypes. This is likely to be caused by phenotypical properties of sin13 at the level of an intrinsic and distinct developmental gradient that occurs within the plant anatomy