Fig. 4

Immunofluorescent labelling of cell wall glycan epitopes in transverse sections from leaves and stems from 4 miscanthus genotypes. Immunolabeling studies were preceded by microscopic inspection of sections stained with toluidine blue to characterise their histological complexity. Monoclonal antibodies (mAbs) used in the study are directed at xyloglucan, MLG, xylan and pectin epitopes. More information on these and other mAbs is provided in Table 1 and in Additional file 1. A base treatment (BT) with 0.1 M KOH, which removed ester-linked substituents from the cell wall, was used together with CCRC-M144 and CCRC-M38. The non-base-treated control of CCRC-M155, as well as the complete immunolabeling study (total of 22 glycan-directed mAbs and 8 genotypes), is available in Additional file 2. ae, abaxial surface epidermis; bs, bundle sheath; on, organelle; gt, parenchymatous ground tissue; is, intercellular space; mf, mesophyll cells; mx, metaxylem; ph, phloem; px, protoxylem; sf, sclerenchyma fibres; st, stomatal complex; xp, xylem parenchyma. Scale bars: 100 µm