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Fig. 2 | Biotechnology for Biofuels

Fig. 2

From: Evolutionary engineering in Saccharomyces cerevisiae reveals a TRK1-dependent potassium influx mechanism for propionic acid tolerance

Fig. 2

Identification of single-nucleotide polymorphism (SNPs) in PA-evolved lineages. After sequencing, Geneious Pro 9.1.3 software was used to map the reads to the CEN.PK113-7D reference genome and subsequently to perform variant calling. Non-synonymous polymorphisms in CDS regions were identified. Mutations that were also present in the parental strain, minimal medium or buffered minimal medium conditions were excluded. The Venn diagram shows that the genes with non-synonymous mutations that were identified from three end-point PA-evolved populations (a). The table presents variant frequencies of Trk1p in three PA-evolved lineages sampled at different generations. G represents the generation number at which the population was sequenced (b)

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