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Fig. 5 | Biotechnology for Biofuels

Fig. 5

From: Evolutionary engineering in Saccharomyces cerevisiae reveals a TRK1-dependent potassium influx mechanism for propionic acid tolerance

Fig. 5

The effect of potassium concentrations and TRK1 on the tolerance of yeast strains to PA. WT, trk1Δ and three end-point-evolved isolates (PA-1, PA-3, and PA-4) were grown to an OD600 = 0.2, serially diluted to 1:0, 1:10, 1:100, 1:1000, and then spotted on YNB agar supplemented with increasing concentrations of potassium (0.1 mM, 1 mM, 10 mM, and 100 mM) with 0 mM, 15 mM, and 25 mM PA. The plates were incubated for 3 days at 30 °C

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