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Table 2 Overexpression of the key genes involving glutamic acid synthesis and secretion on glutamic acid production

From: Engineering Corynebacterium glutamicum triggers glutamic acid accumulation in biotin-rich corn stover hydrolysate

Straina Glutamic acid titer (g/L) Glutamic acid yield (%) Glutamic acid productivity (g L−1 h−1)b
XW6-pH36 59.5 ± 0.5 54.9 ± 0.3 1.36 ± 0.11
XW6-pH36–pyc 55.3 ± 0.3 50.0 ± 0.8 1.38 ± 0.01
XW6-pH36–ppc 55.4 ± 0.4 51.4 ± 1.3 1.39 ± 0.01
XW6-pH36–gltA 60.5 ± 0.1 54.0 ± 0.0 1.26 ± 0.02
XW6-pH36–icd 58.6 ± 0.0 52.7 ± 1.7 1.22 ± 0.00
XW6-pH36–gdh1 56.7 ± 0.7 53.6 ± 0.1 1.18 ± 0.01
XW6-pH36–gdh2 55.8 ± 0.4 52.2 ± 1.2 1.40 ± 0.01
XW6-pH36–ΔC110 59.5 ± 0.5 56.1 ± 0.3 1.49 ± 0.01
  1. Glutamic acid fermentations were carried out in 3-L fermentor (3BG-4, Baoxing Biotech Co., Shanghai, China) at 32 °C, 1.4 vvm of aeration and 600 rpm. No penicillin was added for induction
  2. aKey genes involving glutamic acid synthesis from phosphoenolpyruvate node and glutamic acid secretion were plasmid-based overexpressed in C. glutamicum XW6 (ΔC110odhARBS0.1) strain
  3. bProductivities were calculated based on the maximum glutamic acid titer and the time to reach the maximum glutamic acid titer