Engineering Corynebacterium glutamicum triggers glutamic acid accumulation in biotin-rich corn stover hydrolysate

Table 2 Overexpression of the key genes involving glutamic acid synthesis and secretion on glutamic acid production

Strain^a	Glutamic acid titer (g/L)	Glutamic acid yield (%)	Glutamic acid productivity (g L⁻¹ h⁻¹)^b
XW6-pH36	59.5 ± 0.5	54.9 ± 0.3	1.36 ± 0.11
XW6-pH36–pyc	55.3 ± 0.3	50.0 ± 0.8	1.38 ± 0.01
XW6-pH36–ppc	55.4 ± 0.4	51.4 ± 1.3	1.39 ± 0.01
XW6-pH36–gltA	60.5 ± 0.1	54.0 ± 0.0	1.26 ± 0.02
XW6-pH36–icd	58.6 ± 0.0	52.7 ± 1.7	1.22 ± 0.00
XW6-pH36–gdh1	56.7 ± 0.7	53.6 ± 0.1	1.18 ± 0.01
XW6-pH36–gdh2	55.8 ± 0.4	52.2 ± 1.2	1.40 ± 0.01
XW6-pH36–ΔC110	59.5 ± 0.5	56.1 ± 0.3	1.49 ± 0.01

Glutamic acid fermentations were carried out in 3-L fermentor (3BG-4, Baoxing Biotech Co., Shanghai, China) at 32 °C, 1.4 vvm of aeration and 600 rpm. No penicillin was added for induction
^aKey genes involving glutamic acid synthesis from phosphoenolpyruvate node and glutamic acid secretion were plasmid-based overexpressed in C. glutamicum XW6 (ΔC110odhARBS0.1) strain
^bProductivities were calculated based on the maximum glutamic acid titer and the time to reach the maximum glutamic acid titer

ISSN: 2731-3654