Table 3 Strains and plasmids

E. coli DH5α

Host for plasmid construction

Lab stock

Amorphotheca resinae ZN1

Biodetoxification fungus isolated by our lab

Lab stock

C. glutamicum S9114

Industrial strain for glutamate production

SIIM^a

ΔbioY

In-frame deletion of bioY gene

This study

ΔC110

C-terminal truncation of MscCG

This study

ΔC110ΔbioY

ΔC110 with in-frame deletion of bioY gene

This study

ΔC110odhARBS20

ΔC110 with RBS20 substitution of odhA gene

This study

ΔC110odhARBS10

ΔC110 with RBS10 substitution of odhA gene

This study

ΔC110odhARBS0.1 (XW6)

ΔC110 with RBS0.1 substitution of odhA gene

This study

XW6-pH36

XW6 strain carrying the empty expressing vector

This study

XW6-pH36–pyc

XW6 strain carrying the pyc-expressing vector

This study

XW6-pH36–ppc

XW6 strain carrying the ppc-expressing vector

This study

XW6-pH36–gltA

XW6 strain carrying the gltA-expressing vector

This study

XW6-pH36–icd

XW6 strain carrying the icd-expressing vector

This study

XW6-pH36–gdh1

XW6 strain carrying the gdh1-expressing vector

This study

XW6-pH36–gdh2

XW6 strain carrying the gdh2-expressing vector

This study

XW6-pH36–ΔC110

XW6 strain carrying C-terminal-truncated MscCG expressing vector

This study

XW6-ΔRS02700::H36-ΔC110

XW6 strain with C-terminal-truncated MscCG-encoding gene integrated into the genome at CGS9114_RS02700 locus

This study

pTRCmob

Overexpression vector, kanamycin resistance Km^R

[42]

pK18mobsacB

Mobilizable vector, allows for selection of double crossover in C. glutamicum, kanamycin resistance, sacB

[42]

pH36mob

Overexpression vector derived from pTRCmob

This study

pK18–ΔC110

Plasmid for truncation of C-terminal of MscCG

This study

pK18–ΔbioY

Plasmid for bioY gene knockout

This study

pK18–odhARBS0.1

Plasmid for RBS with 0.1 a.u. substitution of odhA

This study

pK18–odhARBS10

Plasmid for RBS with 10 a.u. substitution of odhA

This study

pK18–odhARBS20

Plasmid for RBS with 20 a.u. substitution of odhA

This study

pK18–ΔRS02700::H36ΔC110

Plasmid for integrating C-terminal-truncated MscCG encoding gene into the genome at CGS9114_RS02700 locus

This study

pH36–pyc

pyc gene-overexpressing vector

This study

pH36–ppc

ppc gene-overexpressing vector

This study

pH36–gltA

gltA gene-overexpressing vector

This study

pH36–icd

icd gene-overexpressing vector

This study

pH36–gdh1

gdh1 gene-overexpressing vector

This study

pH36–gdh2

gdh2 gene-overexpressing vector

This study

pH36–ΔC110

C-terminal-truncated MscCG-encoding gene-overexpressing vector

This study