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Table 1 Bacterial strains and plasmids used in this study

From: Enhancement of lignocellulosic degradation in high-moisture alfalfa via anaerobic bioprocess of engineered Lactococcus lactis with the function of secreting cellulase

Strain and plasmid Relevant trait(s) Source or reference
Strains
 Escherichia coli DH5α supE44 Δlac U169 (Φ80 lacZ ΔM15) hsdR17 recA1, endA1 gyrA96 thi-l relA1 This laboratory
 Lactococcus lactis subsp. lactis MG1363 A plasmid-free derivative of NCDO712; source of usp45; ADT indicator This laboratory
 Trichoderma reesei 3.3711 Type culture China General Microbiological Culture Collection Center
 E. coli DH5α/pMG36e E. coli DH5α with pMG36e This study
 E. coli DH5α/pMG36e-bgl1 E. coli DH5α with pMG36e-bgl1 This study
 E. coli DH5α/pMG36e-cbh2 E. coli DH5α with pMG36e-cbh2 This study
 E. coli DH5α/pMG36e-egl3 E. coli DH5α with pMG36e-egl3 This study
 E. coli DH5α/pMG36e-usp45-bgl1 E. coli DH5α with pMG36e-usp45-bgl1 This study
 E. coli DH5α/pMG36e-usp45-cbh2 E. coli DH5α with pMG36e-usp45-cbh2 This study
 E. coli DH5α/pMG36e-usp45-egl3 E. coli DH5α with pMG36e-usp45-egl3 This study
 E. coli DH5α/pMD18-T-usp45-bgl1 E. coli DH5α with pMD18-T-usp45-bgl1 This study
 E. coli DH5α/pMD18-T-usp45-cbh2 E. coli DH5α with pMD18-T-usp45-cbh2 This study
 E. coli DH5α/pMD18-T-usp45-egl3 E. coli DH5α with pMD18-T-usp45-egl3 This study
 L. lactis subsp. lactis MG1363/pMG36e L. lactis subsp. lactis MG1363 with pMG36e This study
 L. lactis subsp. lactis MG1363/pMG36e-bgl1 L. lactis subsp. lactis MG1363 with pMG36e-bgl1 This study
 L. lactis subsp. lactis MG1363/pMG36e-cbh2 L. lactis subsp. lactis MG1363 with pMG36e-cbh2 This study
 L. lactis subsp. lactis MG1363/pMG36e-egl3 L. lactis subsp. lactis MG1363 with pMG36e-egl3 This study
 L. lactis subsp. lactis MG1363/pMG36e-usp45-bgl1 L. lactis subsp. lactis MG1363 with pMG36e-usp45-bgl1 This study
 L. lactis subsp. lactis MG1363/pMG36e-usp45-cbh2 L. lactis subsp. lactis MG1363 with pMG36e-usp45-cbh2 This study
 L. lactis subsp. lactis MG1363/pMG36e-usp45-egl3 L. lactis subsp. lactis MG1363 with pMG36e-usp45-egl3 This study
Plasmids
 pMD18-T Ampr Takara Biotechnology Co., Ltd.
 pMD18-T-usp45-bgl1 Ampr, clone usp45-bgl1 fusion gene This study
 pMD18-T-usp45-cbh2 Ampr, clone usp45-cbh2 fusion gene This study
 pMD18-T-usp45-egl3 Ampr, clone usp45-egl3 fusion gene This study
 pMG36e Emr; expression vector with the P32 promoter, multiple cloning sites (MCF) and prtP translational terminator Liu et al. [20]
 pMG36e-bgl1 Emr; expression of bgl1 This study
 pMG36e-cbh2 Emr; expression of cbh2 This study
 pMG36e-egl3 Emr; expression of egl3 This study
 pMG36e-usp45-bgl1 Emr, secretory expression of bgl1 This study
 pMG36e-usp45-cbh2 Emr, secretory expression of cbh2 This study
 pMG36e-usp45-egl3 Emr, secretory expression of egl3 This study