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Table 1 Bacterial strains and plasmids used in this study

From: Enhancement of lignocellulosic degradation in high-moisture alfalfa via anaerobic bioprocess of engineered Lactococcus lactis with the function of secreting cellulase

Strain and plasmid

Relevant trait(s)

Source or reference

Strains

 Escherichia coli DH5α

supE44 Δlac U169 (Φ80 lacZ ΔM15) hsdR17 recA1, endA1 gyrA96 thi-l relA1

This laboratory

 Lactococcus lactis subsp. lactis MG1363

A plasmid-free derivative of NCDO712; source of usp45; ADT indicator

This laboratory

 Trichoderma reesei 3.3711

Type culture

China General Microbiological Culture Collection Center

 E. coli DH5α/pMG36e

E. coli DH5α with pMG36e

This study

 E. coli DH5α/pMG36e-bgl1

E. coli DH5α with pMG36e-bgl1

This study

 E. coli DH5α/pMG36e-cbh2

E. coli DH5α with pMG36e-cbh2

This study

 E. coli DH5α/pMG36e-egl3

E. coli DH5α with pMG36e-egl3

This study

 E. coli DH5α/pMG36e-usp45-bgl1

E. coli DH5α with pMG36e-usp45-bgl1

This study

 E. coli DH5α/pMG36e-usp45-cbh2

E. coli DH5α with pMG36e-usp45-cbh2

This study

 E. coli DH5α/pMG36e-usp45-egl3

E. coli DH5α with pMG36e-usp45-egl3

This study

 E. coli DH5α/pMD18-T-usp45-bgl1

E. coli DH5α with pMD18-T-usp45-bgl1

This study

 E. coli DH5α/pMD18-T-usp45-cbh2

E. coli DH5α with pMD18-T-usp45-cbh2

This study

 E. coli DH5α/pMD18-T-usp45-egl3

E. coli DH5α with pMD18-T-usp45-egl3

This study

 L. lactis subsp. lactis MG1363/pMG36e

L. lactis subsp. lactis MG1363 with pMG36e

This study

 L. lactis subsp. lactis MG1363/pMG36e-bgl1

L. lactis subsp. lactis MG1363 with pMG36e-bgl1

This study

 L. lactis subsp. lactis MG1363/pMG36e-cbh2

L. lactis subsp. lactis MG1363 with pMG36e-cbh2

This study

 L. lactis subsp. lactis MG1363/pMG36e-egl3

L. lactis subsp. lactis MG1363 with pMG36e-egl3

This study

 L. lactis subsp. lactis MG1363/pMG36e-usp45-bgl1

L. lactis subsp. lactis MG1363 with pMG36e-usp45-bgl1

This study

 L. lactis subsp. lactis MG1363/pMG36e-usp45-cbh2

L. lactis subsp. lactis MG1363 with pMG36e-usp45-cbh2

This study

 L. lactis subsp. lactis MG1363/pMG36e-usp45-egl3

L. lactis subsp. lactis MG1363 with pMG36e-usp45-egl3

This study

Plasmids

 pMD18-T

Ampr

Takara Biotechnology Co., Ltd.

 pMD18-T-usp45-bgl1

Ampr, clone usp45-bgl1 fusion gene

This study

 pMD18-T-usp45-cbh2

Ampr, clone usp45-cbh2 fusion gene

This study

 pMD18-T-usp45-egl3

Ampr, clone usp45-egl3 fusion gene

This study

 pMG36e

Emr; expression vector with the P32 promoter, multiple cloning sites (MCF) and prtP translational terminator

Liu et al. [20]

 pMG36e-bgl1

Emr; expression of bgl1

This study

 pMG36e-cbh2

Emr; expression of cbh2

This study

 pMG36e-egl3

Emr; expression of egl3

This study

 pMG36e-usp45-bgl1

Emr, secretory expression of bgl1

This study

 pMG36e-usp45-cbh2

Emr, secretory expression of cbh2

This study

 pMG36e-usp45-egl3

Emr, secretory expression of egl3

This study