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Table 3 Kinetic parameters of nonstructural carbohydrates reduction in alfalfa silages based on first-order exponential decay model y = y0 + a*e(−b*x)

From: Enhancement of lignocellulosic degradation in high-moisture alfalfa via anaerobic bioprocess of engineered Lactococcus lactis with the function of secreting cellulase

Additives WSC Glucose Fructose
y0 a b y0 a b y0 a b
Control 8.98e 43.6a 0.69c 0.00b 5.81a 1.99 0.84c 4.20b 1.67a
EN 11.4c 41.3c 0.77b 0.05b 5.76a 1.77 1.28b 3.76c 1.33b
HT1 10.2d 42.3b 0.77b 0.00b 5.81a 2.00 0.73d 4.31a 1.35b
HT1 + EN 12.0b 40.6d 0.73bc 0.04b 5.77a 1.75 1.50a 3.56d 0.65c
HT2 13.2a 39.5e 0.85a 0.13a 5.64b 1.70 1.51a 3.53d 1.35b
SEM 0.014 0.125 0.017 0.015 0.035 0.108 0.010 0.009 0.040
Significance < 0.001 < 0.001 < 0.001 < 0.001 0.004 0.054 < 0.001 < 0.001 < 0.001
  1. Means within a symbol with different superscript lowercase letters (a–e) differ (P < 0.05) according to Tukey’s test
  2. According to Li et al. [28], first-order exponential decay model was y = y0 + ae (−b*x): y (g/kg DM) is the residue at any time x (day); y0 (g/kg DM) is the total residual fraction after 60 days of ensiling; a (g/kg DM) is the consumable fraction; b (day−1) is the fractional consumption rate of a and x is the ensilage time (day)
  3. EN, cellulase; HT1, wild-type L. lactis subsp. lactis MG1363; HT1 + EN, combination of HT1 and EN; HT2, combination of transgenically engineered L. lactis strains HT1/pMG36e-usp45-bgl1, HT1/pMG36e-usp45-cbh2, and HT1/pMG36e-usp45-egl3; SEM, standard error of the means