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Fig. 1 | Biotechnology for Biofuels

Fig. 1

From: AmyZ1: a novel α-amylase from marine bacterium Pontibacillus sp. ZY with high activity toward raw starches

Fig. 1

SDS-PAGE and Native-PAGE analysis of AmyZ1. a SDS-PAGE. M: protein molecular weight marker (Thermo Fisher Scientific, Inc.); lane 1 and 2: the sonication supernatant and precipitate of E. coli harboring plasmid pET22b(+)-AmyZ1 without induction; lane 3 and 4: the sonication supernatant and precipitate of E. coli harboring plasmid pET22b(+)-AmyZ1 induced by IPTG; lane 5: the protein denaturated by 8 M urea; lane 6: the protein after renaturation; lane 7: the target protein after dialysis. b Native-PAGE analysis of the purified AmyZ1. M: native protein molecular weight marker (Thermo Fisher Scientific, Inc.); lane 1: the purified native protein

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