Fig. 3

Effects of CaCl₂ and NaCl on AmyZ1 activity (a, c) and stability (b, d). For the effect on enzyme activity, the assays were carried out at 35 °C with additional supplement of CaCl₂ or NaCl. For the effect on enzyme stability, the assays were performed by incubating the enzyme at 35 °C in Na₂HPO₄–KH₂PO₄ buffer (50 mM, pH 7.0) containing CaCl₂ or NaCl. The residual activities were determined at various time intervals. All the results were the average of triplicate experiments