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Fig. 4 | Biotechnology for Biofuels

Fig. 4

From: AmyZ1: a novel α-amylase from marine bacterium Pontibacillus sp. ZY with high activity toward raw starches

Fig. 4

Optimization of hydrolysis conditon toward raw starches from corn, wheat, and rice. a Substrate concentrations. The reaction mixture contained 20% or 30% raw starches, and 5 U/mg starch of AmyZ1. The assays was performed at 30 °C and 150 rpm for 24 h. b Enzyme dosage. With 30% raw starches as the substrates, the reaction mixture contained 0.5–10 U/mg starch of AmyZ1 as the catalyst. c Reaction temperature. The reaction mixture contained 30% raw starch slurry and appropriate units of AmyZ1. The assays was performed at 20–35 °C. d Time course of the reaction catalyzed by AmyZ1. All the results were the average of triplicate experiments

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