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Table 3 Hydrolysis rates of AmyZ1 and other known α-amylases with high concentration raw starches as the substrates

From: AmyZ1: a novel α-amylase from marine bacterium Pontibacillus sp. ZY with high activity toward raw starches

Source (enzyme name)

Degree of hydrolysis (%)

Enzyme amount (U mg−1 starch)

Temp (°C)

pH

Refs

Wheat

Corn

Bacteria

 Bacillus aquimaris MKSC 6.2 (BaqA)

NM

1.4% (10% and 24 h)

2a

37

6.5

[20]

 Bacillus sp. YX-1

NM

50% (20% and 12 h)

0.3

50

5.0

[53]

 Geobacillus thermoleovorans (Gt-amy)

26% (30% and 3 h)

24% (30% and 3 h)

0.1

60

5.0

[12]

 Geobacillus thermoleovorans (Gt-amyII)

NM

31% (30% and 3 h)

70.0

50

7.0

[18]

 Nesterenkonia sp. strain F

10% (15% and 48 h)

NM

NM

45

NM

[55]

 Geobacillus sp. 4j (Gs4j-amyA)

45% (30% and 4 h)

36% (30% and 4 h)

0.5

65

5.5

[17]

 Bacillus amyloliquefaciens (BAA, Sigma)

50% (30% and 4 h)

15% (30% and 4 h)

0.5

65

5.5

[17]

 Bacillus licheniformis (BLA, Sigma)

50% (30% and 4 h)

35% (30% and 4 h)

0.5

65

5.5

[17]

 Bacillus licheniformis (BLA, Sigma)

NM

58% (30% and 5 h)

11.5

60

6.5

[16]

 Bacillus licheniformis ATCC9945a (BliAmy)

NM

58% (30% and 5 h)

11.5

60

6.5

[16]

 Bacillus licheniformis (BLA, Sigma)

35% (30% and 4 h)

43% (30% and 4 h)

1.0

30

7.0

This study

Pontibacillus sp. ZY (AmyZ1)

37% (30% and 4 h)

47% (30% and 4 h)

5.0b or 1.0c

30

7.0

This study

Fungi and other

 Rhizomucor sp. (RA)

NM

75% (31% and 96 h)d

15.5

32

4.5

[56]

 Porcine pancreas (PPA)

NM

32% (31% and 96 h)d

15.5

37

7.0

[56]

  1. NM not mentioned
  2. aμg/mg
  3. bWith raw wheat starch as the substrate
  4. cWith raw corn starch as the substrate
  5. dTotal solubilized sugars were measured in the supernatant by the orcinol sulfuric method